Journal: bioRxiv

Article Title: Endothelial Sphingosine-1-Phosphate Receptor (S1PR) 1, a Functional S1PR in the Human Cerebrovascular Endothelium, Limits Blood Brain Barrier Permeability and Neuronal Injury following Subarachnoid Hemorrhage in Mice

doi: 10.1101/2025.01.19.633813

Figure Lengend Snippet: HBMVEC were transfected with scrambled (Scr) or S1PR1 siRNA and subjected to normoxia or hypoxia, as indicated in the methods section. A) Hypoxia-induced barrier leakage was exacerbated by S1pr1-knockdown compared to Scr transfected cells. Hypoxia-induced (B, D) phosphorylation of myosin light chain (pMLC) and (C, E) formation of stress fibers was heightened in S1pr1 siRNA transfected cells compared to Scr. B) IF staining for pMLC. C) Stress fiber formation was visualized by phalloidin staining. (B, C) Representative images from N=3-6 independent trials conducted in duplicates. Quantification was done using BioTek Lionheart automated imaging module Gen5. The data points and the mean ± SEM are plotted. *P < 0.05; **P < 0.01; ***P < 0.001; **** P < 0.0001; two-way ANOVA multiple comparisons followed by Tukey’s.

Article Snippet: For stress fiber detection, cells were incubated with fluorescent phalloidin (1:400 in PBS; Invitrogen) with emission at 549nm.

Techniques: Transfection, Knockdown, Staining, Imaging

Journal: Frontiers in Cell and Developmental Biology

Article Title: Human Derived Immortalized Dermal Papilla Cells With a Constant Expression of Testosterone Receptor

doi: 10.3389/fcell.2020.00157

Figure Lengend Snippet: Detection of cytoskeletal F-actin in wild type, K4DT, AR expressing K4DT DPCs. (A) Staining feature of wild type, K4DT, and AR expressing K4DT DPCs. Staining with phalloidin, DAPI, and merged pictures were presented. (B) The quantitation of fluorescence intensity from the cytoplasm of 15 randomly selected cells. Average and standard error were shown in the graph. No significant difference.

Article Snippet: After the permeabilized treatment with Triton X-100, we stained the cells with X40 diluted Rohodamine X conjugated Phalloidin solution (code 165-2164, FUJIFILM Wako Pure Chemical, Osaka, Japan, powder was dissolved into 1.5 mL of dimethylformamide, final concentration is 6.6 μmol/L).

Techniques: Expressing, Staining, Quantitation Assay, Fluorescence

Journal: Cytotechnology

Article Title: Effects of recombinant baculovirus AcMNPV- Bm K IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells

doi: 10.1007/s10616-014-9789-x

Figure Lengend Snippet: Formation of early cables of actin in AcMNPV and AcMNPV-BmK IT infected Sf9 cells. a Uninfected cells as a mock were fixed in 4 % paraformaldehyde. Then, the actin filaments were stained with the F-actin-specific stain rhodamine-phalloidin and the cellular DNA in the nuclei was stained with DAPI. b, c Cells were infected with AcMNPV and AcMNPV-BmK IT with a multiplicity of ten infectious particles per cell in 250 μl of medium and fixed in 4 % paraformaldehyde at 2 and 3 h p.i., respectively, and then stained with rhodamine-phalloidin and DAPI. The actin cytoskeleton was visualized with Delta Vision RT. Scale bar 50 μm

Article Snippet: Rhodamine phalloidin stock solution (Cytoskeleton, Inc., Denver, CO, USA): the lyophilized product was reconstituted to a 14 μM solution with 500 μM of 100 % methanol.

Techniques: Infection, Staining

Journal: Cytotechnology

Article Title: Effects of recombinant baculovirus AcMNPV- Bm K IT on the formation of early cables and nuclear polymerization of actin in Sf9 cells

doi: 10.1007/s10616-014-9789-x

Figure Lengend Snippet: Formation of nuclear F-actin in AcMNPV and AcMNPV-BmK IT infected Sf9 cells. a–g Cells were infected with AcMNPV and AcMNPV-BmK IT with a multiplicity of ten infectious particles per cell in 250 μl of medium and fixed in 4 % paraformaldehyde at 6, 12, 21, 25, 31, 37 and 46 h p.i., respectively, then stained with rhodamine-phalloidin and DAPI. The actin cytoskeleton was visualized with Delta Vision RT. Scale bar 50 μm

Article Snippet: Rhodamine phalloidin stock solution (Cytoskeleton, Inc., Denver, CO, USA): the lyophilized product was reconstituted to a 14 μM solution with 500 μM of 100 % methanol.

Techniques: Infection, Staining